Bioanalytical method development, validation and quantification of Bosentan by high-performance liquid chromatography in rat plasma was written by Nallakumar, P.;Siva, Kumar R.. And the article was included in World Journal of Pharmaceutical Research in 2017.Category: oxazolidine This article mentions the following:
A Simple and rapid bioanal. high performance liquid chromatog. (HPLC) method for the determination of Bosentan using Losertan as an internal standard was developed and validated as per regulatory requirements. Sample preparation was accomplished through liquid phase extraction and chromatog. separation on a reverse phase column. The mobile phase consists of mixture of methanol and water in the ratio of 50:50 at a flow rate of 1ml/min. The wavelength used for the detection of bosentan was 225 nm with a total run time of 6 min. The retention times of bosentan and losartan were found to be 2 and 4 resp. The method was developed and tested for the linearity range of 250-750mg/mL. The method was validated for accuracy, precision, linearity and recovery in compliance to international regulatory guidelines. In the experiment, the researchers used many compounds, for example, 5-((3,5-Dimethylphenoxy)methyl)oxazolidin-2-one (cas: 1665-48-1Category: oxazolidine).
5-((3,5-Dimethylphenoxy)methyl)oxazolidin-2-one (cas: 1665-48-1) belongs to oxazolidine derivatives. Oxazolidines are well known as key portions of bioactive molecules or precursors of chiral molecules, as well as established chiral auxiliaries. As well as other multifunctional heterocyclic compounds, oxazolidine rings play an essential role in organic and medicinal chemistry, behaving, in some cases as powerful antitumor agents.Category: oxazolidine
Referemce:
Oxazolidine – Wikipedia,
Oxazolidine | C3H7NO – PubChem