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Primary amine hydrochlorides promote a well-controlled ring-opening polymerisation of Z-L-lysine-N-carboxyanhydride in DMF at 40-80 degrees C; the polystyrene-poly(Z-L-lysine) block copolymers synthesised exhibit a very narrow molecular weight distribution, close to a Poisson distribution.

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An A-B-C type triblock copolymer, tandemly aligning two types of polycations with different pKa values in a single polymer strand, was developed for the construction of novel polyplex micelles, satisfying a high DNA condensing ability as well as a proton buffering activity directed to elevating gene transfection. The micelle might feature the distinctive three-layered structure, where an inner polyplex layer of condensed pDNA with poly(l-lysine) (pKa ?9.4) as the C segment is successively wrapped with an intermediate layer of poly[(3-morpholinopropyl)aspartamide] (B segment) with a comparatively low pKa of?6.2, to provide a buffering effect, and an outer PEG layer (A segment) as a biocompatible palisade. Copyright

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An all in one nano-system with active-targeting, enzyme-triggered deshielding and positive-charge characteristics was fabricated for chemo/photo-combination therapy to allow efficient tumor targeting, cellular internalization and lysosomal escape. The deshielding of NPs was induced by enzyme triggered degradation of the NP shell, and consequently exposure of the positively charged core accelerates escape of NPs from the lysosome to exert anticancer effects with high efficiency.

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Self-assembling block copolypeptides were prepared by sequential ring-opening polymerization of N-carboxyanhydride (NCA) derivatives of g-benzyl-L-glutamic acid and e-carbobenzyloxy-L-lysine, followed by selective deprotection of the benzyl glutamate block. The synthesized polymers had number average molecular weights close to theoretical values, and had low dispersities (DM =1.15-1.28). Self-assembly of the amphiphilic block copolymers into nanoparticles was achieved using the “solvent-switch” method, whereby the polymer was dissolved in THF and water and the organic solvent removed by rotary evaporation. The type of nanostructures formed varied from spherical micelles to a mixture of spherical and worm-like micelles, depending on copolymer composition. The spherical micelles had an average diameter of 43nm by dynamic light scattering, while the apparent diameter of the mixed phase system was around 200nm. Reproducibility of nanoparticle preparation was demonstrated to be excellent; almost identical DLS traces were obtained over three repeats. Following qualitative dye-solubilization experiments, the nanoparticles were loaded with the ocular anti-inflammatory drug dexamethasone. Loading efficiency of the nanoparticles was 90% and the cumulative drug release was 94% over 16d, with a20% burst release in the first 24h.

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Primary amine hydrochlorides promote a well-controlled ring-opening polymerisation of Z-L-lysine-N-carboxyanhydride in DMF at 40-80 degrees C; the polystyrene-poly(Z-L-lysine) block copolymers synthesised exhibit a very narrow molecular weight distribution, close to a Poisson distribution.

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Polypeptides have attracted widespread attention as building blocks for complex materials due to their ability to form higher-ordered structures such as beta-sheets. However, the ability to precisely control the formation of well-defined beta-sheet-assembled materials remains challenging as beta-sheet formation tends to lead to ill-defined and unprocessable aggregates. This work reports a simple, rapid, and robust strategy to form well-defined peptide beta-sheet-assembled shells (i.e., hollow spheres) by employing surface-initiated N-carboxyanhydride ring-opening polymerization under a highly efficient surface-driven approach. The concept is demonstrated by the preparation of enzyme-degradable rigid shell architectures composed of H-bonded poly(L-valine) (PVal) grafts with porous and sponge-like surface morphology. The porous PVal-shells exhibit a remarkable and unprecedented ability to non-covalently entrap metal nanoparticles, proteins, drug molecules, and biorelevant polymers, which could potentially lead to a diverse range of biodegradable and functional platforms for applications ranging from therapeutic delivery to organic catalysis.

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Poly(L-lysine) hydrobromide in water was coated onto a platinum electrode and then doped with ferrocene residues by reaction with ferrocenecarbonyl chloride in benzene containing pyridine.The behaviour of this coating to cyclic voltammetry in acetonitrile was examined.There is no evidence of the ageing phenomenon previously reported for poly(Nepsilon-4-nitrobenzoyl-L-lysine) films.In the unchanged state, ferrocene residues are associated.In the oxidised state, the ferrocinium residues appear evenly distributed through the film.Reduction of the oxidised state yields afilm in which the ferrocene residues are evenly distributed but which relaxes within a few minutes to the state where ferrocene residues are associated.Charging can be repeated a large number of times with no deterioration in response.

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The, invention belongs. to the, field of pharmaceutical preparations, RAGE and relates to a multi-target treatment micelle for regulating and ROS controlling a microenvironment of’s disease and a preparation method thereof, and, RAGE, AD; AD, ROS, AD. (by machine translation)

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Coloring compositions comprising peptide-based dispersants and/or binders are provided. The compositions are particularly useful for the coloring or dyeing of substrates such as paper and textile fabrics. The peptide-based dispersant and/or binder compositions are distinguished by the presence of at least one positively charged terminal amino acid on the peptide portion of the composition which enhances binding to the substrate.

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A composition and method capable of delivering pharmaceutical or biomedical materials includes a tri-block surfactant having a hydrophilic block, a charged water-soluble block and a hydrophobic block.

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